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KMID : 0613820070170121723
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Journal of Life Science
2007 Volume.17 No. 12 p.1723 ~ p.1728
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Effect of Antioxidant Activity and Induction of DNA Damage on Human Gastric Cancer Cell by Rubus coreanus Miquel
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Jeon Sang-Kyung
Lee Ji-Won
Lee In-Seon
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Abstract
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Rubus coreanus Miquel (RCM), a type of red raspberry, grows wild in Korea and China and its unripe fruit is used as a folk medicine for the treatment of impotence and as a diuretic. RCM was extracted with methanol and then further fractionated it into for different types. In this study, we investigated the antioxidant activity of a RCM extract (ext.) and its fraction (fr.). DPPH free radical scavenging activity assay, total polyphenols contents, total flavonoids contents assay were used to analyze antioxidant activity. The DPPH free radical scavenging activity (RC50: 1.67 μg/ml) and total polyphenols contents (546.25 μg/mg) were higher in butanol fraction than in other fr. And total flavonoids contents was higher in ethylacetate fr. (141.78 μg/mg). We applied comet assay to measure the DNA damage in the individual cells and exposed time course at IC50. Comet assay is a rapid and sensitive fluorescent microscopic method to examine DNA damage and repair at individual cell level. The butanol fr. from RCM significantly induced 54.12%, 57.95% of DNA damage after treated RCM for 8 hr. In conclusion, we found that RCM has antioxidant activity and DNA damage effects on gastric cancer cells.
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KEYWORD
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Rubus coreanus Miquel, comet assay, antioxidant activity, DNA damage, gastric cancer cells
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